In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are precipitated as a complex. Other interfering materials are also presumably removed by absorption to the protein-sulphosalicylic acid complex. The extracted proline is made to react with ninhydrin in acidic conditions (pH 1.0) to form the [...]
In this article we will discuss about the gravimetric method for estimation of sulphate in plants. Principle: Sulphate is precipitated as barium sulphate in hydrochloric acid medium by the addition of barium chloride solution. The reaction is carried out near the boiling temperature. The precipitate is filtered and washed to remove the chlorides, then dried or ignited and weighed as [...]
In this article we will discuss about the Lowry’s method for estimation of protein in plants. Principle: The blue colour developed by the reduction of the phosphomolybdic-phosphotungstic components in the Folin-Ciocalteau reagent by the amino acids tyrosine and tryptophan present in the protein plus the colour developed by the biuret reaction of the protein with the alkaline cupric tartrate are [...]