In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are precipitated as a complex. Other interfering materials are also presumably removed by absorption to the protein-sulphosalicylic acid complex. The extracted proline is made to react with ninhydrin in acidic conditions (pH 1.0) to form the [...]
In this article we will discuss about the gravimetric method for estimation of sulphate in plants. Principle: Sulphate is precipitated as barium sulphate in hydrochloric acid medium by the addition of barium chloride solution. The reaction is carried out near the boiling temperature. The precipitate is filtered and washed to remove the chlorides, then dried or ignited and weighed as [...]
In this article we will discuss about the top two methods used for estimation of sugar in plants. The methods are: 1. Titrimetric Method 2. Colorimetric Method Using Anthrone Reagent. Method # 1. Titrimetric Method: Principle: All the sugars with free reducing group (aldehyde or ketone) undergo enolisation in presence of alkali. As a result they behave as strong reducing [...]