Let us make an in-depth study of the meaning, categories, principles and protocols of embryo culture. What is Embryo Culture? The embryo of different developmental stages, formed within the female gametophyte through sexual process, can be isolated aseptically from the bulk of maternal tissues of ovule, seed or capsule and cultured in vitro under aseptic and controlled physical conditions in [...]
The following points highlight the five important methods employed for culturing single cells. The methods are: (1) The Paper Raft Nurse Technique (2) The Petri Dish Plating Technique (3) The Micro-chamber Technique (4) The Nurse Callus Technique and (5) The Micro-droplet Technique. Method # 1. The Paper Raft Nurse Technique: 1. Single cells are isolated from suspension cultures or a [...]
Let us make an in-depth study of the meaning, principle, factors affecting and importance of single cell culture. What is the Meaning of Single Cell Culture? Single cell culture is a method of growing isolated single cell aseptically on a nutrient medium under controlled condition. Principle of Single Cell Culture: The basic principle of single cell culture is the isolation [...]