In this article we will discuss about the preparation of different stains used in microscopy. 1. Acetocarmine (Semi chon): Reagents: 45% glacial acetic acid … 100 ml Carmine powder … 10 g Preparation: Mix the two; heat up to boiling. Cool, filter and store for future use. 2. Borax Carmine: Reagents: Aqueous borax solution … 4%. Carmine solution … 3%. [...]
In this article we will discuss about the study of non-chordatas under microscope. 1. Amoeba sp.: Collection: It is a fresh water protozoan. Collect water from a stagnant pond with a little bottom mud, floating scum, slime, along with the stems and leaves of aquatic weeds. Put all those in a glass jar and leave undisturbed for a few days. [...]
In this article we will discuss about the dissection of different contents for vertebrates and invertebrates under microscope. I. Gut Contents: Preparation: Cut a small portion of the gut, contents of which is to be studied. Put it in a watch glass. Open it giving a longitudinal incision with a pair of fine scissors. Add a small amount of physiological [...]