In this article we will discuss about the artificial synthesis of gene. For the first time in 1955, Michelson chemically synthesized a dinucleotide in laboratory. Later on in 1970, Har Govind Khorana and K.L. Agarwal for the first time chemically synthesized gene coding for tyrosine tRNA of yeast. For the synthesis of tRNA and rRNA there are specific genes. However, [...]
In this article we will discuss about the approaches made for codon assignments. 1. Codon Assignment with Unknown Sequences: Theoretically it was considered that genetic code should be triplet so that codons must be assigned for 20 amino acids. But it was not known which codon synthesizes which amino acid. Therefore, the first approach was made to find out the [...]
In this article we will discuss about the concept of wobble hypothesis. Crick (1966) proposed the 'wobble hypothesis' to explain the degeneracy of the genetic code. Except for tryptophan and methionine, more than one codons direct the synthesis of one amino acid. There are 61 codons that synthesise amino acids, therefore, there must be 61 tRNAs each having different anticodons. [...]