Transgenic plants expressing vaccine antigens can be used to tackle autoimmune disease like diabetes. Insulin dependent diabetes melites is an autoimmune disease resulted in the destruction of insulin producing pancreatic cells. Disease is triggered by involvement of both humoral and cell mediated immunity. The type I diabetes by autoimmunity is due to the inadvert attack on insulin producing cells by [...]
In this article we will discus about the models for post-transcriptional gene silencing (PTGS). Production of dsRNA from inverted transgene repeats and single copy transgene triggers PTGS. It was proposed that dsRNA would be act as template for an RNA-directed RNA polymerase. Atleast four models have been proposed to explain induction of PTGS. First model is known as threshold model [...]
In this article we will discuss about: 1. Methods of Cloning, 2. Cloning of a Specific Gene, 3. Cloning of a Specific Gene, 4. Expression of Cloned Genes, 5. Cloning of Genes in Eucaryotic Cells and 6. Precautions to be taken during Experiments of Genetic Recombination. Methods of Cloning: In the past few years, an increasing number of laboratories have [...]