In this article we will discuss about:- 1. Isolation of DNA to be Cloned 2. Insertion of Foreign DNA Fragment into a Vector 3A. Transfer of Recombinant DNA into Bacterial Cell 3B. Cloning in Eukaryotes 4. Detection of Recombinant Clone. Gene Cloning Technique # 1. Isolation of DNA to be Cloned: The DNA of interest, i.e., target DNA may [...]
The below mentioned article provides a study note on molecular mapping. Preparation of linkage map based on recombination data is always handicapped due to non-availability of mutants for many genes. This limitation has largely been overcome in recent years by molecular mapping through ISH (in situ hybridization), Restriction mapping, RFLP (Restriction Fragment Length Polymorphism) analysis, RAPD (Random Amplified Polymorphic DNA) [...]
Genetic engineering is considered as a kind of biotechnology. This is a process in which the alteration of the genetic make-up of cells is done by deliberate and artificial means. This process involves transfer or replacement of genes to create recombinant DNA. This can be done by cutting DNA molecules at specific sites to get fragments containing desirable and useful [...]