In this article we will discuss about:- 1. Introduction to PCR 2. Technique of PCR 3. Modified Forms. Introduction to PCR: PCR provides a simple and ingenious method for exponential amplification of specific DNA sequences by in vitro DNA synthesis, i.e., this technique has made it possible to synthesize large quantities of DNA fragments without cloning it. Kary Mulis in [...]
In this article we will discuss about the Gene Transfer Techniques used in Plants:- 1. Introduction to Gene Transfer Techniques 2. Vectors of Gene Transfer. Introduction to Gene Transfer Techniques: The aim of plant genetic engineering is to transfer a foreign gene to a recipient species to alter the genome of the recipient and to give a new characteristic of [...]
The following methods highlight the top three methods used in gene sequencing. The methods are: 1. Maxam & Gilbert's Chemical Degradation Method 2. Sanger and Coulson's Dideoxynucleotide Synthetic Method 3. Direct DNA Sequencing using PCR. 1. Maxam & Gilbert's Chemical Degradation Method: In this method the following steps are involved (Fig. 18.17) i. The 3' ends of DNA fragments are [...]