In this article we will discuss about:- 1. Introduction to PCR 2. Technique of PCR 3. Modified Forms. Introduction to PCR: PCR provides a simple and ingenious method for exponential amplification of specific DNA sequences by in vitro DNA synthesis, i.e., this technique has made it possible to synthesize large quantities of DNA fragments without cloning it. Kary Mulis in [...]
In this article we will discuss about the Gene Transfer Techniques used in Plants:- 1. Introduction to Gene Transfer Techniques 2. Vectors of Gene Transfer. Introduction to Gene Transfer Techniques: The aim of plant genetic engineering is to transfer a foreign gene to a recipient species to alter the genome of the recipient and to give a new characteristic of [...]
The following methods highlight the two important methods of gene transfer used in plants. The methods are: 1. Indirect Method 2. Direct Method. 1. Indirect Method: Transfer via Agrobacterium: The transforÂmation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in legumes. This bacterium carries a plasmid with [...]