The following points highlight the top two methods of gene transfer. The methods are: 1. Indirect Method (Agrobacterium Mediated Gene Transfer) 2. Direct Methods of Gene Transfer. Method # 1. Indirect Method (Agrobacterium Mediated Gene Transfer): The transformation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in [...]
In this article we will discuss about the methods of gene transfer. Gene transfer can be done by two methods: (i) Indirect Method through Vectors or Carriers and (ii) Direct or Vectorless Transfer Method. The first method includes: (a) Plasmids and Viruses and (b) Natural Genetic Engineer, (a) Plasmids and Viruses: Recombinant DNA technology involves the transfer of desired genes [...]
This article throws light upon the top four methods of gene transfer. The top four methods of gene transfer are: (1) DNA Transfer in Protoplasts (2) Free DNA Transfer to Intact Tissue (3) Agrobacterium Mediated Gene Transfer Method and (4) Integration and Expression. The first transgenic plant was produced via Agrobacterium mediated modified transformation of Nicotiana tabacum protoplasts by Horsch [...]