In this article we will discuss about the Insertion of a Foreign DNA Fragment into a Vector. Introduction to the Insertion of a Foreign DNA Fragment into a Vector: The specific section of DNA generated by the action of restriction enzyme is spliced or incorporated into an agent called cloning vehicle. Cloning vehicle is also called vector. Cloning vehicles or [...]
The following points highlight the top three methods of recombinant DNA formation. The methods are: 1. Transformation 2. Transfection 3. Non-Bacterial Transformation. Recombinant DNA Formation: Method # 1. Transformation: The restriction enzyme which causes a break in foreign DNA also causes a staggered cut in the vector DNA at a specific cleavage site. The cohesive ends of vector DNA possess [...]
In this article we will discuss about the Multiplication of Host Cell and Cloning of Genes. Introduction to Multiplication of Host Cell and Cloning of Genes: The host cell containing foreign DNA in addition to its own DNA is allowed to multiply forming a clone having millions of identical cells. Each member of the clone contains in addition to its [...]