The below mentioned article provides a study note on DNA. Nature of DNA: DNA is a very long polymer of purine and pyrimidine mononucleotide monomers bound one to the other by phosphodiester bridges. It exists as a double-stranded molecule in nature. The strands are held together by Vander Waals' forces and the purine and pyrimidine bases of the two strands [...]
In this article we will discuss about the Polymerase Chain Reaction:- 1. Steps of Polymerase Chain Reaction 2. Advantages of Polymerase Chain Reaction 3. Applications. Steps of Polymerase Chain Reaction: PCR uses DNA polymerase to amplify repetitively targeted portions of DNA. The amplified DNA sequence can then be analysed by southern hybridization. i. Primer Construction: (a) It is essential to [...]
The below mentioned article provides a study note on the Prenatal Diagnosis of Genetic Diseases. It is required to analyse DNA of family members of the affected individual to set up a diagnostic tool for a genetic disease. The markers (RFLPs) that are tightly linked to the disease is needed to be identified first. The physicians are required to have [...]