In this article we will discuss about the tests for estimation of peroxidase in plants. Principle: The enzyme peroxidase catalyses the oxidation of substrate by oxygen generated from the decomposition of hydrogen peroxide: 2H2O2 → 2H2O + O2 Substrate + O2 → Oxidized substrate. Requirements: (a) Reagents: 1. Hydrogen peroxide (H2O2) — 0.2% 2. Catechol soln. — 0.5% (b) Glass-wares: [...]
In this article we will discuss about the colorimetric method for estimation of phosphorus in plants. Principle: When ammonium molybdate solution is added to a solution of phosphate containing conc. H2SO4 it produces a yellow crystalline precipitation of ammonium phospho-molybdate. Phospho-molybdate reacts with amino-naphthol-sulphonic acid and produces a molybdenum complex which forms a blue- coloured solution. The appearance of colour [...]
In this article we will discuss about the tests for estimation of lipase in plants. Principle: The quantity of fatty acid released in unit time is measured by the quantity of NAOH required to maintain pH constant. The milliequivalent of alkali consumed is taken as a measure of the activity of the enzyme. Materials: a. Substrate: Take 2 ml of [...]