In this article we will discuss about the tests for estimation of acid phosphatase in plants. Principle: The enzyme phosphatase hydrolyzes p-nitro-phenol phosphate. The released p-nitro-phenol is yellow in colour in alkaline medium and is measured at 405 nm. The optimum pH for acid and alkaline phosÂphatases are 5.3 and 10.5, respectively. Materials: (i) Sodium Hydroxide 0.085 N: Dissolve 0.85 [...]
In this article we will discuss about the tests for estimation of acetylcholine esterase in plants. Principle: The hydrolysed acetylcholine per unit time is measured by comparison of the initial concentration in a reference tube with the final concentration in the experimental tube. Acetylcholine is made to react with hydroxylamine to form the corresponding acylhydroxamic acid which forms a strongly [...]
In this article we will discuss about the tests for estimation of polyphenol oxidase enzyme activity. Principle: The intensely yellow 2-nitro-5-thio-benzoic acid (TNB) with an absorption maximum at 412 nm reacts with quinones generated through enzymatic oxidation of 4-methyl-catechol (catechol oxidase) and 1, 4 di-hydroxy-benzene (laccase) to yield colourless adducts. The decrease in the absorbance of yellow- colour due to [...]