In this article we will discuss about the areas and scope of plant biotechnology. The term 'Biotechnology' was coined in 1917 by a Hungarian Engineer, Karl Ereky, to describe a process for large scale production of pigs. The word biotechnology indicates the interaction between the science of biology and technology. Biotechnology has been broadly defined as, 'the development and utilisation [...]
In this article we will discuss about the methods and success of plant biotechnology. Methods in Plant Biotechnology: i. Tissue and Cell Culture: This technique helps in maintaining plant tissues for prolonged periods on an artificial culture/basal medium. Small group of cells is isolated and inoculated on a culture medium comprising carbohydrate source, vitamins, minerals and defined nitrogenous source(s) under [...]
This article throws light upon the top six methods of DNA polymorphism of a genome. The methods are: 1. RFLP 2. RAPD 3. OAF 4. AP-PCR 5. AFLP 6. SSR. DNA Polymorphism of a Genome: Method # 1. RFLP: Restriction fragment length polymorphism. It is non-PCR based technique where genomic DNA is digested with restriction enzyme(s). The whole genetic material [...]