In this article we will discuss about the global status and commercialised biotech crops. This summarisation is based on James (2009) where Clive James has dedicated the publication to the late Nobel Peace Laureate Norman Borlaug, first founding patron of ISAAA (The International Service for the Acquisition of Agri-biotech Applications). The global status of commercialized biotech/GM/transgenic crops can be plainly [...]
In this article we will discuss about the DNA extraction and amplification. Normally, CTAB (cetyl trimethyl ammonium bromide) method is used. However, SDS method is also common and is described as follows: 1. The leaves are washed with sterilised distilled water and blot dried and cut into small pieces with a sterilised blade. 2. 1g of leaf material is placed [...]
Most molecular markers fall into one of three basic categories of techniques that use either hybridization or are based on the polymerase chain reaction (PCR): Category # 1: Hybridization Based (non-PCR) Techniques: Category I includes restriction fragment length polymorphism (RFLP) analysis, where probes are hybridized to filters containing DNA which has been digested with restriction enzymes. The resultant fragments are [...]