Archive | Protein Purification

This article throws light upon the four methods of protein purification. The four methods of protein purification are: (1) Extraction (2) Precipitation and Differential Solubilisation (3) Ultracentrifugation and (4)Chromatographic Methods. The methods used in protein purification, can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein, that [...]

Read this article to learn about the meaning, principle strategies, selection and combination of purification techniques, purification of a tagged protein, evaluation of purification yield, concentration of purified protein and analysis of isolated proteins. Introduction: Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the [...]