Archive | Flowcytometry

Principles of Flowcytometry Data Analysis (With Diagram)

Read this article to learn about the principles of flowcytometry data analysis. Data Analysis: Gates and Regions: An important principle of flow cytometry data analysis is to selectively visualize the cells of interest while eliminating results from unwanted particles, e.g., dead cells and debris. This procedure is called gating. Cells have traditionally been gated according to physical characteristics. For instance, [...]

By |2015-10-16T05:52:04+00:00October 10, 2015|Flowcytometry|Comments Off on Principles of Flowcytometry Data Analysis (With Diagram)

Principles of Fluorescence (With diagram)

Read this article to learn about the principles of fluorescence. Fluorochromes and Light: Fluorochromes are essentially dyes, which accept light energy (e.g., from a laser) at a given wave­length and re-emit it at a longer wavelength. These two processes are called excitation and emission. The process of emission follows extremely rapidly, commonly in the order of nanoseconds, and is known [...]

By |2015-10-16T05:53:10+00:00October 10, 2015|Flowcytometry|Comments Off on Principles of Fluorescence (With diagram)

Principles of Flowcytometry (With Diagram)

Read this article to learn about the principles of flowcytometry. Introduction: The fluorescent antibody techniques described are extremely valuable qualitative tools, but they do not give quantitative data. This shortcoming was remedied by development of the flow cytometer, which was designed to automate the analysis and separation of cells stained with fluorescent antibody. The flow cytometer uses a laser beam [...]

By |2015-10-16T05:53:54+00:00October 10, 2015|Flowcytometry|Comments Off on Principles of Flowcytometry (With Diagram)
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