Read this article to learn about the general properties of electrophoresis. The term electrophoresis describes the migration of a charged particle under the influence of an electric field. Many important biological molecules, such as amino acids, peptides, proteins, nucleotides and nucleic acids, possess ionisable groups and, therefore, at any pH, exist in solution as electrically charged species either as cations [...]
Read this article to learn about the detection, estimation and recovery of protein gels. The most commonly used general protein strain for detecting protein on gels is the sulphate trim-ethylamine dye Coomassie Brilliant Blue R-250 (CBB). Staining is usually carried out using 0.1% (w/v) CBB in methanol: water: glacial acetic acid (45:45:10, by vol.). This acid-methanol mixture acts as a [...]
This article throws light upon the top ten types of electrophoretic techniques used in biochemistry. The ten types of electrophoretic techniques used in biochemistry are: (1) Horizontal and Vertical Gel Electrophoresis Systems (2) Agarose Gel Electrophoresis (3) Polyacrylamide Gels (4) Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (5) Native (Buffer) Gels (6) Gradient Gels (7) Capillary Electrophoresis (8) Cellulose Acetate Electrophoresis (9) [...]