This article throws light upon the five principal methods used for creation of transgenic animals. The five principal methods are: (1) Physical Transfection (2) Chemical Transfection (3) Retrovirus-Mediated Gene Transfer (4) Virus Vector and (5) DNA Packaged inside a Bacterium. Method # 1. Physical Transfection: This method involves the direct microinjection of a chosen gene construct (a single gene or [...]
Read this article to learn about the production of transgenic animals using virus as a vector. A review of the literature shows that to date the only successful method to produce transgenic rabbits, pigs, sheep, goats and cattle is DNA-microinjection technique into the pro-nucleus. Rabbits: Rabbits are used as experimental models in gene transfer experiments. In 1985, the successful production [...]
This article throws light upon the three methods used for introducing a foreign gene into transgenic mice. The three methods are: (1) Retroviral Vector Method (2) Microinjection Method and (3) Embryonic Stem Cell Method. Methods for Introducing a Foreign Gene: Method # 1. Retroviral Vector Method: The transfer of small pieces (8 kb) of DNA can be effectively carried out [...]