The following points highlight the top three ways for separating amino acids from peptides. The ways are: 1. Paper Chromatography 2. Electrophoresis 3. Ion Exchange Chromatography. Separating Amino Acids from Peptides: Way # 1. Paper Chromatography: a. A small volume (about 0.005 ml containing about 0.01 mg) of amino acid is applied at a marked point 5 cm from the [...]
The below mentioned article provides a study note on Amino Acids:- 1. Common Properties of Amino Acids 2. Physical Properties of Amino Acids 3. Chemical Reactions. Common Properties of Amino Acids: 1. Pro-tonic Equilibria of Amino Acids: (a) Amino acids have at least two ionizable groups i.e. -COOH and –NH+3. The former dissociates more easily than the latter. In solution, [...]
Let us learn about Separation of Amino Acids by Paper Chromatography . A mixture of unknown amino acids can be separated and identified by means of paper chromatography. The position of the amino acids in the chromatogram can be detected by spraying with ninhydrin, which reacts with amino acids to yield highly coloured products (purple). Paper chromatography is a method [...]