In this article, we will discuss about the nucleoside and nucleotide. A nucleoside is composed of two components: (a) Nitrogenous base and (b) Five-carbon sugar (pentose). 1. Nitrogenous bases: The nitrogenous bases are derivatives of two parent heterocyclic compounds, i.e., purine and pyrimidine. 2. Pentose Sugars: The pentose sugar present in the nucleic acids is ribose in RNA and deoxyribose [...]
Let us make an in-depth study of the nucleic acids. The below given article will help you to learn about the following things:- 1. DNA 2. Structure of DNA 3. Chemical Composition of DNA 4. Nitrogen Bases 5. Nucleosides and Nucleotides 5. Polymerization 6. Genetic Information in DNA 7. Chargaff Equivalence Rule 8. Types of DNA and Others. In 1868 [...]
Read this article to learn about the isolation and separation, electrophoresis and blotting method of nucleic acid. Isolation and Separation of Nucleic Acids: Isolation of DNA: DNA is recovered from cells by the gentlest possible method of cell rupture to prevent DNA from fragmenting by mechanical shearing. This is usually in the presence of EDTA which chelate the Mg2+ needed [...]