In this article we will discuss about the meaning and some methods of double staining. Meaning of Staining: Staining is essential because different tissues and other structures can be easily differentiated by staining. The plant materials, in which there is no differentiation of tissues such as members of algae, fungi and bryophyta, are stained by a single staining process. But [...]
The following points highlight the top two staining techniques for recognition of bacteria. The techniques are: 1. Gram Stain Method 2. Ziehl Neelsen Method. Staining Technique # 1. Gram Stain Method: Before staining, the position of the smear on the slide should be confirmed by making a scratch with the help of a needle or sharp glass slide, NEVER with [...]
The following points highlight the top three staining methods used for colouring the biological materials. The types are: 1. Negative Staining 2. Simple Staining 3. Differential Staining. Method # 1. Negative Staining: For quicker observation of the morphology of bacterial or yeast cells, the negative staining technique is applied. Requirements: 1. Bacterial/yeast suspension. 2. Slide, inoculating needle, pipettes etc. 3. [...]