Fusion of protoplasts can be accomplished by two distinct methods like chemical fusion and electro-fusion (Fig. 10.1). Earliest method known as mechanical fusion, where protoplasts are forced to fuse under applied pressure within the pipette. Protoplast in suspension can undergo spontaneous fusion in cultural conditions. Method # 1. Chemical Fusion of Protoplast: Chemicals such as sodium nitrate, polyethylene glycol and [...]
Generally, isolation of protoplast is categorized into mechanical and enzymatic. Also learn about the techniques involved in the isolation of leaf mesophyll protoplast. Mechanical Method: This method is crude and oldest method for protoplast isolation. It is only an historical perspective. It involves chopping and plasmolysis of tissues. Time consuming and low yield of protoplast are the main constraints in [...]
The below mentioned article provides an overview on the Isolation and Culture of Protoplast:- 1. Protocol for Isolation and Culture of Protoplast 2. Isolation of Protoplast from Cell Suspension Culture 3. Haploid Protoplast 4. Protoplast from PMC and Pollen Tetral 5. Tests for Viability 6. Wall Formation, Cell Division and Callus Formation 7. Plant Regeneration and Others. Contents: Protocol for [...]