The below mentioned article provides study notes on Microtome Sections:- 1. Section-Cutting 2. Mounting of Sections 3. Stretching of Sections 4. Drying of Sections 5. Staining the Sections 6. Staining the Sections 7. Schedule of Staining the Section. Microtome Sections # Section-Cutting: Rotary microtomes are commonly used for section-cutting. Move the block holding the socket backward as far as possible [...]
In this article we will discuss about the preparation of paraffin block. Transfer the tube or tubes containing the material to a high temperature paraffin bath (60°C), along with some paraffin of suitable melting point taken in a beaker. Suitable melting point refers to the melting point of paraffin which will give ideal ribbons of sections (see section cutting). This [...]
In this article we will discuss about the types and advantages of using microtome. Types of Microtomes: The inherent defects of handmade sections can be eliminated by using a microtome. Three types of microtomes are available: Rotary, Rocking and Sliding Microtomes (Fig. 3.1). A Rotary microtome is worked by rotating a wheel fitted with a handle. The razor is placed [...]