In this article we will discuss about the Lowry’s method for estimation of protein in plants. Principle: The blue colour developed by the reduction of the phosphomolybdic-phosphotungstic components in the Folin-Ciocalteau reagent by the amino acids tyrosine and tryptophan present in the protein plus the colour developed by the biuret reaction of the protein with the alkaline cupric tartrate are [...]
In this article we will discuss about the top two methods used for estimation of sugar in plants. The methods are: 1. Titrimetric Method 2. Colorimetric Method Using Anthrone Reagent. Method # 1. Titrimetric Method: Principle: All the sugars with free reducing group (aldehyde or ketone) undergo enolisation in presence of alkali. As a result they behave as strong reducing [...]
In this article we will discuss about the tests for estimation of polyphenol oxidase enzyme activity. Principle: The intensely yellow 2-nitro-5-thio-benzoic acid (TNB) with an absorption maximum at 412 nm reacts with quinones generated through enzymatic oxidation of 4-methyl-catechol (catechol oxidase) and 1, 4 di-hydroxy-benzene (laccase) to yield colourless adducts. The decrease in the absorbance of yellow- colour due to [...]