In this article we will discuss about the tests for the detection of alkaloids in plants with the help of experiment. The alkaloids are a group of basic, secondary plant substance, which usually possess a N-containing hetero-cycle. Their basic character is due to this feature. The alkaloids are mostly colourless, crystalline and non-volatile solids. They are insoluble in water, but [...]
Enzymes are thermo-labile organic catalysts which are very much sensitive to heat and pH. The activity of a number of respiratory enzymes could be detected within plant tissue by suitable tests. (a) Detection of Dehydrogenase: This enzyme accomplishes intracellular oxidation and reduction by the transfer of hydrogen from one type of molecule to another. The importance of such enzyme is [...]
In this article we will discuss about the gravimetric method for estimation of sulphate in plants. Principle: Sulphate is precipitated as barium sulphate in hydrochloric acid medium by the addition of barium chloride solution. The reaction is carried out near the boiling temperature. The precipitate is filtered and washed to remove the chlorides, then dried or ignited and weighed as [...]