In this article we will discuss about the tests for the detection of alkaloids in plants with the help of experiment. The alkaloids are a group of basic, secondary plant substance, which usually possess a N-containing hetero-cycle. Their basic character is due to this feature. The alkaloids are mostly colourless, crystalline and non-volatile solids. They are insoluble in water, but [...]
Enzymes are thermo-labile organic catalysts which are very much sensitive to heat and pH. The activity of a number of respiratory enzymes could be detected within plant tissue by suitable tests. (a) Detection of Dehydrogenase: This enzyme accomplishes intracellular oxidation and reduction by the transfer of hydrogen from one type of molecule to another. The importance of such enzyme is [...]
In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are precipitated as a complex. Other interfering materials are also presumably removed by absorption to the protein-sulphosalicylic acid complex. The extracted proline is made to react with ninhydrin in acidic conditions (pH 1.0) to form the [...]