In this article we will discuss about some physico-chemical principles which plays a great role in physiology with the help of suitable diagrams. Also learn the physiological importance of each physico-chemical principles. 1. Filtration: Filtration is the process by which un-dissolved particles are separated from a liquid through a membrane as a result of a mechanical force (filtering force). It [...]
Object: To separate leaf pigments by column chromatography. or To separate the chloroplast pigments by solvent method. Requirements: Pigments extract, magnesium oxide, filter glass disc, glass wool, flask, suction pump, chromatographic tube, petroleum ether, benzene. Method: 1. Take a chromatographic tube having a filter glass disc and glass wool at its base. 2. Fill the chromatographic tube with magnesium oxide. [...]
Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take 4 clean microscopic slides and [...]