In this article we will discuss about:- 1. General Properties of Plasmids 2. Types of Plasmids 3. Replication 4. Incompatibility 5. Library. General Properties of Plasmids: Plasmids are defined as extra-chromosomal genetic elements, occurring chiefly in bacteria and rarely in eukaryotic organisms. In bacteria, plasmids are circular double-stranded DNA molecules which contain genes controlling a wide variety of functions. In [...]
A genome library consists of a large collection of bacterial cultures, in each of which a different segment of the genomic DNA of an organism has been cloned by recombinant DNA technology, so that the entire collection represents its complete genome. The analogy between a library of books and a genome library is based on the fact that both genes [...]
In eukaryotic organisms — plants, animals and fungi — the major portion of DNA is present in the chromosomes which are well-organised structures and quite different from the prokaryotic counterparts. Besides the chromosomes, mitochondria of both plants and animals and the chloroplasts of green plants also contain DNA. Interesting is the fact that the organization and the nature of the [...]