In this article we will discuss about the techniques used for measuring cell numbers and cell mass of microorganisms. Measurement (Count) of Cell Numbers: 1. Breed Method: A known volume of microbial cell suspension (0.01 ml) is spread uniformly over a glass slide covering a specific area (1 sq. cm). The smear is then fixed by heating, stained, examined under [...]
The following points highlight the six main physical factors affecting the growth of microorganisms. The factors are: 1. Solutes and Water Acidity 2. Temperature 3. pH 4. Oxygen Requirements 5. Pressure 6. Radiation. Factor # 1. Solutes and Water Acidity: Water is one of the most essential requirements for life. Thus, its availability becomes most important factor for the growth [...]
Learn about the comparison between moist heat sterilization and dry heat sterilization. Microorganisms are killed by heat as a result of the inactivation of their proteins (including enzymes) and, as stated earlier, the heat is applied either in moist or in dry conditions in processes of sterilization called moist heat sterilization and dry heat sterilization, respectively. The conditions and mechanisms [...]