Immune system is a defense system that enables us to resist infections. The immune system is composed of two types of immunity- the innate or non-specific immunity and the adaptive or specific immunity. The innate immunity is the first line of defense against invading organisms while the adaptive immunity acts as a second line of defense and also gives protection [...]
Antigen (Ag) antibody (Ab) reactions occur when an antigen combines with a corresponding antibody to produce an immune complex. Therefore, an antigen-antibody reaction is thus a bimolecular association which is similar to an enzyme-substrate interaction but the only difference is that antigen-antibody reaction does not lead to an irreversible chemical interaction. The basis for antigen-antibody reactions are the non-covalent interactions [...]
In this article we will discuss about:- 1. Assay Development 2. Assay Evaluation 3. Assay Validation. Assay Development: The main developmental goals of an assay system are its desired accuracy, precision, sensitivity and specificity. Therefore, the foremost consideration in an assay development and optimization should be the intended application of the assay which may further affect the developmental goals. Immunoassays [...]