Tag Archives | Human Genetics

Top 8 Methodologies Neces­sary for Genetic Research

The following points highlight the top eight methodologies necessary for genetic research. The methodologies are:- 1. Isolation of Plasmids 2. Isolation of Chromosomal DNA 3. Agarose Gel Electrophoresis 4. Restriction and Ligation 5. Transformation 6. Polyacrylamide Gel Electrophoresis 7. Two-Dimensional Electrophoresis and 8. Immunoelectrophoresis. Methodology # 1. Isolation of Plasmids: Plasmids are self-replicating, extrachro-mosomal genetic elements found in bacteria. They [...]

By |2016-12-12T07:34:25+00:00December 12, 2016|Human Genetics|Comments Off on Top 8 Methodologies Neces­sary for Genetic Research
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Genetic Code: Aspects and Mechanisms | Human Genetics

In this article we will discuss about the functional aspects and mechanisms of genetic code. Functional Aspects of Genetic Codon: All the amino acids except methionine and tryptophan are specified by more than one codon. Three amino acids—leucine, serine and orginine—are each specified by six differ­ent codons. Isoleucine has three codons, and the other amino acids each have either two [...]

By |2016-12-12T07:34:24+00:00December 12, 2016|Genetic Code|Comments Off on Genetic Code: Aspects and Mechanisms | Human Genetics
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Enzyme Linked Immunosorbent Assay (EIA) | Human Genetics

The below mentioned article provides notes on Enzyme Linked Immunosorbent Assay (EIA). Enzyme immunoassay (EIA) was first described by Engvall and Perlmann (1971, 1972), and by von Weemen and Schuurs (1971, 1972). The technique was derived from the work of Nakane and Pierce (1967) which showed that antibodies could be labelled with enzymes for use in histochemical staining of tissues [...]

By |2016-12-12T07:34:24+00:00December 12, 2016|Human Genetics|Comments Off on Enzyme Linked Immunosorbent Assay (EIA) | Human Genetics
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