Tag Archives | Human Genetics

Top 8 Methodologies Neces­sary for Genetic Research

The following points highlight the top eight methodologies necessary for genetic research. The methodologies are:- 1. Isolation of Plasmids 2. Isolation of Chromosomal DNA 3. Agarose Gel Electrophoresis 4. Restriction and Ligation 5. Transformation 6. Polyacrylamide Gel Electrophoresis 7. Two-Dimensional Electrophoresis and 8. Immunoelectrophoresis. Methodology # 1. Isolation of Plasmids: Plasmids are self-replicating, extrachro-mosomal genetic elements found in bacteria. They [...]

By |2016-12-12T07:34:25+00:00December 12, 2016|Human Genetics|Comments Off on Top 8 Methodologies Neces­sary for Genetic Research
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Enzyme Linked Immunosorbent Assay (EIA) | Human Genetics

The below mentioned article provides notes on Enzyme Linked Immunosorbent Assay (EIA). Enzyme immunoassay (EIA) was first described by Engvall and Perlmann (1971, 1972), and by von Weemen and Schuurs (1971, 1972). The technique was derived from the work of Nakane and Pierce (1967) which showed that antibodies could be labelled with enzymes for use in histochemical staining of tissues [...]

By |2016-12-12T07:34:24+00:00December 12, 2016|Human Genetics|Comments Off on Enzyme Linked Immunosorbent Assay (EIA) | Human Genetics
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Mechanism of Eukaryotic Gene Expression | Genetics

In this article we will discuss about the mechanism of eukaryotic gene expression, particularly in humans. 1. In multicellular organism, each cell expresses a subset of its genes. 2. A cell expresses different genes depending on its growth state or environment. 3. Sometimes gene transcripts are spliced differently in different cells. 4. Each gene carries not only structural infor­mation but [...]

By |2016-12-12T07:34:24+00:00December 12, 2016|Gene Expression|Comments Off on Mechanism of Eukaryotic Gene Expression | Genetics
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