Growth is defined as an orderly increase in cellular components. Microorganisms grow in a variety of physical and chemical environments. To distinguish orderly growth with that of in orderly growth, in recent years the term "balanced growth" has been used. "Balanced growth" as defined by Campbell, is doubling of every biochemical unit of the cell within the time duration of [...]
In this article we will discuss about the techniques used for measuring cell numbers and cell mass of microorganisms. Measurement (Count) of Cell Numbers: 1. Breed Method: A known volume of microbial cell suspension (0.01 ml) is spread uniformly over a glass slide covering a specific area (1 sq. cm). The smear is then fixed by heating, stained, examined under [...]
The following points highlight the six main physical factors affecting the growth of microorganisms. The factors are: 1. Solutes and Water Acidity 2. Temperature 3. pH 4. Oxygen Requirements 5. Pressure 6. Radiation. Factor # 1. Solutes and Water Acidity: Water is one of the most essential requirements for life. Thus, its availability becomes most important factor for the growth [...]