Geneticists use following two methods for sequencing the genomes: 1. Clone-by-clone Method 2. Shotgun Cloning. Method # 1. Clone-by-Clone Method: This method was the first to be developed. It begins with the construction of genomic libraries of restriction fragments covering all the genomic DNA (or genomic clones) of an organism. By using genetic markers, overlapping clones are assembled into genetic [...]
In this article we will discuss about the concept and development of bioinformatics. Concept of Bioinformatics: Bioinformatics or life science informatics has emerged as a new branch of biotechnology, offering a fundamental tool to the biologist to accelerate commercialization of biotechnology. Bioinformatics is the classic example of convergence of biotechnology and information technolÂogy. Bioinformatics has been the most powerful tools [...]
In this article we will discuss about the analysis of the human genome. Molecular analyses of medical relevance rely heavily on four strategic features: (1) The ability to clone and characterize nucleic acid sequences, (2) The specificity of nucleic acid hybridization, (3) The specificity of restriction endonucleases, and (4) The power of DNA amplification using the polymerase chain reacÂtion (PGR). [...]