Tag Archives | Gene Sequencing

Study Notes on Gene Sequencing | Genetics

The below mentioned article provides a study note on gene sequencing. When a particular gene of interest or a DNA fragment is isolated, the sequencing of that piece of DNA becomes essential. The sequence of DNA refers to the order of nucleotide bases along its sugar phosphate backbone. No technique can determine the sequence of bases in an entire gene [...]

By |2016-11-02T05:49:25+00:00November 2, 2016|Gene Sequencing|Comments Off on Study Notes on Gene Sequencing | Genetics
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Top 3 Methods used in Gene Sequencing

The following methods highlight the top three methods used in gene sequencing. The methods are: 1. Maxam & Gilbert's Chemical Degrada­tion Method 2. Sanger and Coulson's Dideoxynucleotide Synthetic Method 3. Direct DNA Sequencing using PCR. 1. Maxam & Gilbert's Chemical Degrada­tion Method: In this method the following steps are involved (Fig. 18.17) i. The 3' ends of DNA fragments are [...]

By |2016-07-14T18:34:53+00:00July 14, 2016|Genetic Engineering|Comments Off on Top 3 Methods used in Gene Sequencing
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