After reading this article you will learn about the central dogma of molecular biology, with help of a suitable diagram. Crick's Central Dogma: DNA, deoxyribonucleic acid, carries the information necessary for an organism to grow and develop. This information is housed deep within the nucleus of a cell in genes - highly- specific sequences of nucleotides, the building blocks of [...]
In this article we will discuss about:- 1. Gene Expression in Vegetative Organs 2. Gene Expression in Reproductive Organs 3. Gene Expression in Seeds 4. Gene Expression in Fruits. Successful studies on gene expression in plants are undoubtedly due to the contribution of early botanical monograph in which cytological structure and function of organs were defined. With the advent of [...]
Blotting technique is an extremely powerful tool for analyzing gene structure and used to study gene expression, once cloned cDNA is isolated. There are three important types of blotting techniques are: 1. Southern Blotting 2. Northern Blotting 3. Western Blotting. Technique # 1. Southern Blotting: Developed by E.M. Southern, the technique of Southern blotting is one of the most important [...]