Fusion of Protoplast: 2 Methods | Biotechnology
Fusion of protoplasts can be accomplished by two distinct methods like chemical fusion and electro-fusion (Fig. 10.1). Earliest method known as mechanical fusion, where protoplasts are forced to fuse under applied pressure within the pipette. Protoplast in suspension can undergo spontaneous fusion in cultural conditions. Method # 1. Chemical Fusion of Protoplast: Chemicals such as sodium nitrate, polyethylene glycol and [...]