Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take 4 clean microscopic slides and [...]
Object: To demonstrate the phenomenon of plasmolysis. Requirements: Tradescantia leaf, water, sugar solution, slides, cover glass, microscope, blade. Method: 1. From the lower surface of the leaf of Tradescantia, peel off small segments of epidermis by a blade. 2. Put few peelings on a slide, mount in a drop of water, put a cover glass and study under microscope. 3. [...]
The below mentioned article includes a list of three experiments on air pollution. 1. Experiment to measure dust fall in any specified area: Requirements: Dust fall trappers, distilled water, physical balance, sieve, scale. Dust fall: Measurement of dust-fall provides a rough estimate of air pollution. The most common method used is the Dust-trap method, which has a sensitivity up to [...]