Object: To demonstrate the phenomenon of diffusion. Requirements: Beaker, water, copper sulphate crystals. Method: Take a clean beaker and fill it completely with water. Place a big crystal of copper sulphate on one side of the beaker (Fig. 1). Observations: After some time, crystal of copper sulphate disappears and its particles get equally distributed throughout the water. Results: Molecules of [...]
Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take 4 clean microscopic slides and [...]
Object: To demonstrate the phenomenon of plasmolysis. Requirements: Tradescantia leaf, water, sugar solution, slides, cover glass, microscope, blade. Method: 1. From the lower surface of the leaf of Tradescantia, peel off small segments of epidermis by a blade. 2. Put few peelings on a slide, mount in a drop of water, put a cover glass and study under microscope. 3. [...]