Estimation of Protein by Lowry’s Method | Plants

In this article we will discuss about the Lowry’s method for estimation of protein in plants. Principle: The blue colour developed by the reduction of the phosphomolybdic-phosphotungstic components in the Folin-Ciocalteau reagent by the amino acids tyrosine and tryptophan present in the protein plus the colour developed by the biuret reaction of the protein with the alkaline cupric tartrate are [...]