Estimation of Polyphenol Oxidase Enzyme Activity
In this article we will discuss about the tests for estimation of polyphenol oxidase enzyme activity. Principle: The intensely yellow 2-nitro-5-thio-benzoic acid (TNB) with an absorption maximum at 412 nm reacts with quinones generated through enzymatic oxidation of 4-methyl-catechol (catechol oxidase) and 1, 4 di-hydroxy-benzene (laccase) to yield colourless adducts. The decrease in the absorbance of yellow- colour due to [...]