Estimation of Peroxidase | Enzyme Activity
In this article we will discuss about the tests for estimation of peroxidase in plants. Principle: The enzyme peroxidase catalyses the oxidation of substrate by oxygen generated from the decomposition of hydrogen peroxide: 2H2O2 → 2H2O + O2 Substrate + O2 → Oxidized substrate. Requirements: (a) Reagents: 1. Hydrogen peroxide (H2O2) — 0.2% 2. Catechol soln. — 0.5% (b) Glass-wares: [...]