Estimation of Amino Acids | Plants

In this article we will discuss about the colorimetric method for estimation of amino acids in plants. Principle: Amino acid content can be estimated quantitatively by Colorimetric method using ninhydrin reagent. The reaction of ninhydrin (triketohydrindene hydrate) with amino acids is of particular importance in the regard. Ninhydrin is a powerful oxidizing agent and elicits the oxidative deamination of the [...]