DNA Replication in Bacteria | Genetics

The work of J. Cairns demonstrated DNA replication in bacteria in an elegant way. He used high resolution autoradiography for observing the distribution of radioactive label in the "chromosomes" of dividing E. coli cells. The bacterial cells were grown on a medium containing tritiated thymidine. The labelled DNA was extracted from the cells and spread on EM grids. A very [...]