Extraction and Estimation of Deoxyribonucleic Acid (DNA)
In this article we will discuss about the Extraction and Estimation of Deoxyribonucleic Acid (DNA). Principle: Extraction of DNA is accomplished by the rupturing of cell wall and nuclear membrane followed by deproteinization and precipitation of the nucleic acid using ethanol. Materials: a. Sample Material b. Extraction Medium 0-15 M NaCl, 0-1 M Na2 c. Lysozyme solution, 10 mg/ml d. [...]