Practical Experiment on Culturing of Lambda (λ) Phage
The below mentioned article provides a Practical Experiment on Culturing of Lambda (λ) Phage. Principle: Bacteriophage λ is cultured in E.coli cell suspension. The phage is allowed to harbour and multiply till the complete lysis of bacterial cells. Then the bacterial cells are killed with chloroform and the phage particles are separated by centrifugation. Materials: A. E. coli LE 392 [...]