Experiment on Column Chromatography | Botany

Object: To separate leaf pigments by column chromatography. or To separate the chloroplast pigments by solvent method. Requirements: Pigments extract, magnesium oxide, filter glass disc, glass wool, flask, suction pump, chromatographic tube, petroleum ether, benzene. Method: 1. Take a chromatographic tube having a filter glass disc and glass wool at its base. 2. Fill the chromatographic tube with magnesium oxide. [...]