The below mentioned article provides an overview on Cells and Organisms Used in Molecular Biology. For any molecular biological work or genetic engineering, the synthesis and cloning of any recombinant DNA involves introduction of this DNA into bacteria. Structure of E. coli: Among bacteria, escherichia coli are suitable for this purpose for the following reasons: i. It can be grown [...]
The following points highlight the four main replications of DNA. The replications are: 1. Plasmid 2. Mitochondrial DNA 3. Kinetoplast DNA 4. Chloroplast DNA. Replication # 1. Plasmid: In addition to the nucleoid DNA, the cytoplasm of bacterial cell contains one or more extra chromosomal mini-circular DNA duplexes which are known as plasmids that can replicate independently of the nucleoid [...]
Study notes on Fidelity of Replication! Fidelity of replication means the faithful replication of DNA and the production of accurate daughter DNA using the parental DNA as a template. The accuracy of DNA replication is critical to cell reproduction. To produce a sequence of bases in the DNA of a daughter cell which is identical to that in the parent, [...]