Whole blood is diluted with a 3% acetic acid solution, which hemolyzes mature erythrocytes and facilitates leukocyte counting. The standard dilution for leukocyte counts is 1:20. This dilution is prepared using the leukocyte Unopette system. The dilution is mixed well and incubated to permit lysis of the erythrocytes. Following the incubation period, the dilution is mounted on a hemocytometer. The [...]
It is necessary to determine haematocrit with 6 hours after the blood collection. Principle: Blood is centrifuged in sealed capillary tube and PCV is determined by a special haematocrit reader. Requirements: Haematocrit Centrifuge: 1. It runs at high speed and produces RCF of 12,000 X g and runs at a speed of about 15,000RPM. It contains a head to hold [...]
Get the answer of: How is RBC count done ? It is a quantitative measure of the population of blood cells in circulation. The counting of cells in circulation. The counting of cells can be done mainly with the help of a microscope after diluting the blood and making a wet mount. This technique is known as Hemocytometry. Now a [...]