In this article we will discuss about the mechanisms of enzymatic reactions. While studying the kinetics of enzymatic reactions we noted that Michaelis was led to postulate the formation of an intermediate enzyme-substrate complex right at the beginning of this century. What was then only a hypothesis was later largely confirmed and proved. The great disproportion between the size of [...]
In this article we will discuss about the free energy of activation and the role of catalysts. An exergonic reaction is a spontaneous reaction; it can therefore yield energy usable for chemical, mechanical or electrical work. However, it should be remembered that spontaneous does not mean rapid, but only without external energy input. A spontaneous exergonic reaction can be extremely [...]
We will first consider the effects of the variations of two physical factors, temperature and pH. We will then examine certain chemical agents (or effectors) which can modify the kinetics of enzymatic reactions and are either activators or inhibitors. 1. Effect of Temperature: The study of the initial velocity of an enzymatic reaction as a function of temperature reveals two [...]