In this article we will discuss about the replication of ssDNA phage. I. Adsorption: The cell wall surface of E.coli contains specific receptor sites. For example, the receptor site of ØX114 present on outer membrane of cell wall of Salmonella typhimurium is a lipopolysaccharide. The phage gets adsorbed on bacterial cell surface through any one of 12 icosahedral vertices. The [...]
Lambda consists of two types of life cycles, the lytic and lysogenic. Lytic Cycle: The events of lytic cycle, starting with adsorption, at 37°C occurs as below: Time (t) = 0 minutes: Phage adsorbed and DNA is injected = 3 minutes: First early mRNA is synthesized = 5 minutes: Two classes of early mRNA are synthesized = 6 minutes: DNA [...]
The following points highlight the six main therapies of bacteriophages. The therapies are: 1. Collection 2. Mode of Action of Phage Preparations 3. Commercial Production of Phages 4. Prophylaxis and Treatment of Bacterial Infections 5. Characteristics of Bacterlophages 6. Bio-Safety. Therapy # 1. Collection: Local samples of bacteriophage can be collected from water which possibly contains high quantities of bacteria [...]