In this article we will discuss about the growth curve of bacteriophage. One-step growth curve, developed by Max Delbriick and Emory Ellis (1939), marks the starting of modern bacteriophage research. In this experiment, susceptible bacterium (E. coli) is mixed with bacteriophage (T2) and is allowed for a short period to get attached with the host cells. The culture is then [...]
In this article we will discuss about Bacteriophage:- 1. Introduction to Bacteriophage 2. Morphology of Bacteriophage 3. Life Cycle 4. Significance. Introduction to Bacteriophage: Bacteriophage (Greek phagein—to eat; bacteriophage, bacteria-cater) are viruses that infect and parasitise bacteria. They cause lysis of bacteria. They are abbreviated as phages. Twart (1915) and d'Herelle (1917) observed an invisible minute parasite of bacteria that [...]
The below mentioned article provides a Practical Experiment on Culturing of Lambda (λ) Phage. Principle: Bacteriophage λ is cultured in E.coli cell suspension. The phage is allowed to harbour and multiply till the complete lysis of bacterial cells. Then the bacterial cells are killed with chloroform and the phage particles are separated by centrifugation. Materials: A. E. coli LE 392 [...]