The below mentioned article provides a Practical Experiment on Culturing of Lambda (λ) Phage. Principle: Bacteriophage λ is cultured in E.coli cell suspension. The phage is allowed to harbour and multiply till the complete lysis of bacterial cells. Then the bacterial cells are killed with chloroform and the phage particles are separated by centrifugation. Materials: A. E. coli LE 392 [...]
In this article we will discuss about the Structure of Bacterial Cell. Bacteria (sing. bacterium) are unicellular prokaryotic microorganisms which divide by binary fission. They do not possess nuclear membrane and the nucleus consists of a single chromosome of circular double-stranded DNA helix (Fig. 1.1). Flagella: These are long filamentous, cytoplasmic appendages, 12-30 μm in length, protruding through the cell [...]
The following points highlight the two main methods of morphological study of bacteria. The methods are: 1. Unstained Wet Mount 2. Stained Films. Method # 1. Unstained Wet Mount: Drops of liquid specimens of 3-6 hours growth in fluid media at room temperature (22°C) are examined mainly for study of bacterial motility. The method is also used for demonstration of [...]